rabbit anti sult1a1 antibody  (Bioss)

Journal: Nutrients

Article Title: Excess Folic Acid Supplementation before and during Pregnancy and Lactation Alters Behaviors and Brain Gene Expression in Female Mouse Offspring

doi: 10.3390/nu14010066

Figure Lengend Snippet: Sequences of qRT-PCR primers.

Article Snippet: The membrane was blocked with 5% skim milk in Tris-buffered saline (TBS, 150 mM NaCl, 50 mM Tris-HCl, pH 7.4) for 30 min and incubated with rabbit anti-Sult1a1 antibody (1:500, bs-6283R, Bioss Antibodies, Beijing, China) overnight at room temperature.

Techniques: Sequencing, Amplification

Journal: Nutrients

Article Title: Excess Folic Acid Supplementation before and during Pregnancy and Lactation Alters Behaviors and Brain Gene Expression in Female Mouse Offspring

doi: 10.3390/nu14010066

Figure Lengend Snippet: DEGs enriched in the GO category of cellular component were validated by qRT-PCR. The relative mRNA expression level of Tlr1 , Sult1a1 , Tph2 , Acacb , Ppara , Etnppl , Angptl4 and Apold1 genes in control and 2.5 × FA brains ( n = 3) were determined by qRT-PCR. The levels of ribosomal protein S18 (RPS18) mRNA were used for normalization. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Article Snippet: The membrane was blocked with 5% skim milk in Tris-buffered saline (TBS, 150 mM NaCl, 50 mM Tris-HCl, pH 7.4) for 30 min and incubated with rabbit anti-Sult1a1 antibody (1:500, bs-6283R, Bioss Antibodies, Beijing, China) overnight at room temperature.

Techniques: Quantitative RT-PCR, Expressing

Journal: Nutrients

Article Title: Excess Folic Acid Supplementation before and during Pregnancy and Lactation Alters Behaviors and Brain Gene Expression in Female Mouse Offspring

doi: 10.3390/nu14010066

Figure Lengend Snippet: The protein levels of Sult1a1 were elevated in 2.5 × FA brains at P21d. ( A ) The brains of control and 2.5 × FA mice were lysed and analyzed by Western blots, comparing anti-Sult1a1 levels ( n = 6). GAPDH was used as a loading control. ( B ) The protein levels of Sult1a1 were calculated by normalizing to GAPDH. ***, p < 0.001.

Article Snippet: The membrane was blocked with 5% skim milk in Tris-buffered saline (TBS, 150 mM NaCl, 50 mM Tris-HCl, pH 7.4) for 30 min and incubated with rabbit anti-Sult1a1 antibody (1:500, bs-6283R, Bioss Antibodies, Beijing, China) overnight at room temperature.

Techniques: Western Blot

Journal: Molecular Medicine Reports

Article Title: Quercetin-3-O-β-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats

doi: 10.3892/mmr.2018.9249

Figure Lengend Snippet: Summary of the gene-specific PCR primer sequences, the length of production and the appropriate annealing temperature used in the experiments.

Article Snippet: A rabbit anti-SULT1A1 polyclonal antibody (1:1,000; cat. no. bs-6283R) was purchased from Bioss (Beijing, China).

Techniques:

Journal: Molecular Medicine Reports

Article Title: Quercetin-3-O-β-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats

doi: 10.3892/mmr.2018.9249

Figure Lengend Snippet: Effect of Q3GA on the intestinal and hepatic mRNA expression levels of Cyp3a1, Cyp3a2, Ugt1a1, Sult1a1, Gstm1, Slco2b1, Slco1b2, Mdr1, Bcrp, Mrp2, Pxr, and Car. In rats of the control and Q3GA-L, Q3GA-M, Q3GA-H, KET and VER pretreatment groups, the relative mRNA content was measured by reverse transcription-quantitative polymerase chain reaction. mRNA expression levels of (A) Cyp3a1, (B) Cyp3a2, (C) Ugt1a1, (D) Sult1a1, (E) Gstm1, (F) Slco2b1/Slco1b2, (G) Mdr1, (H) Bcrp, (I) Mrp2, (J) Pxr, and (K) Car were measured in the small intestine and liver of the rats. Values are expressed as the mean ± standard deviation (n=3). aP<0.05, bP<0.01, cP<0.001 vs. the control. dP<0.05, eP<0.01, fP<0.001 vs. the inhibitor group (KET/VER). Q3GA-L, low-dose Q3GA (2.5 mg/kg); Q3GA-M, middle-dose Q3GA (5 mg/kg); Q3GA-H, high-dose Q3GA (10 mg/kg). CsA, cyclosporin A; Q3GA, quercetin-3-O-β-D-glucoside; KET, ketoconazole; VER: Verapamil; Cyp, cytochrome P450; Gstm, glutathione-S-transferase-µ; Q3GA, quercetin-3-O-β-D-glucoside; Slco, solute carrier organic anion transporter family member; Sult, sulfotransferase; Car, constitutive androstane receptor; Mdr1, multidrug resistance protein 1; Mrp2; multidrug resistance protein 2; Pxr, pregnane X receptor; Bcrp, breast cancer resistance protein.

Article Snippet: A rabbit anti-SULT1A1 polyclonal antibody (1:1,000; cat. no. bs-6283R) was purchased from Bioss (Beijing, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Quercetin-3-O-β-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats

doi: 10.3892/mmr.2018.9249

Figure Lengend Snippet: Effect of Q3GA on the intestinal and hepatic protein expression levels of CYP3A1, CYP3A2, UGT1A1, SULT1A1, GSTM1, OATP2B1, OATP1B2, P-gp, BCRP, MRP2, PXR, and CAR. In rats of the control and Q3GA-L, Q3GA-M, Q3GA-H, KET, VER pretreatment groups, the relative protein contents were assessed by western blotting. Representative western blotting results in (A) liver and (B) in small intestine. Western blotting results of (Aa) UGT1A1, SULT1A1, GSTM1, OATP1B2, BCRP, MRP2, PXR and CAR, (Ab) CYP3A1/2 and (Ac) P-gp in liver. Western blotting results of (Ba) UGT1A1, SULT1A1, GSTM1, OATP2B1, BCRP, MRP2, PXR and CAR, (Bb) CYP3A1/2 and (Bc) P-gp in small intestine. Q3GA-L, low-dose Q3GA (2.5 mg/kg); Q3GA-M, middle-dose Q3GA (5 mg/kg); Q3GA-H, high-dose Q3GA (10 mg/kg). BCRP, breast cancer resistance protein; CAR, constitutive androstane receptor; CsA, cyclosporin A; CYP, cytochrome P450; GSTM, glutathione-S-transferase-µ; KET, ketoconazole; MRP, multidrug resistance-associated protein; OATP1B1, organic anion transporting polypeptide 1B1; P-gp, P-glycoprotein; PXR, pregnane X receptor; Q3GA, quercetin-3-O-β-D-glucoside; SULT, sulfotransferase; UGT, UDP glucuronosyltransferase; VER, verapamil.

Article Snippet: A rabbit anti-SULT1A1 polyclonal antibody (1:1,000; cat. no. bs-6283R) was purchased from Bioss (Beijing, China).

Techniques: Expressing, Western Blot

Journal: Molecular Medicine Reports

Article Title: Quercetin-3-O-β-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats

doi: 10.3892/mmr.2018.9249

Figure Lengend Snippet: Quantification of the effect of Q3GA on the intestinal and hepatic protein levels, assessed by western blotting. Quantification of western blotting results revealed protein expression levels of (A) CYP3A1, (B) CYP3A2, (C) UGT1A1, (D) SULT1A1, (E) GSTM1, (F) OATP2B1/OATP1B2, (G) P-gp, (H) BCRP, (I) MRP2, (J) PXR, and (K) CAR measured in the small intestine and liver of rats. Values are expressed as the mean ± standard deviation (n=3). aP<0.05, bP<0.01, cP<0.001 vs. control; dP<0.05, eP<0.01, fP<0.001 vs. inhibitor group (KET/VER). Q3GA-L, low-dose Q3GA (2.5 mg/kg); Q3GA-M, middle-dose Q3GA (5 mg/kg); Q3GA-H, high-dose Q3GA (10 mg/kg). BCRP, breast cancer resistance protein; CAR, constitutive androstane receptor; CsA, cyclosporin A; CYP, cytochrome P450; GSTM, glutathione-S-transferase-µ; KET, ketoconazole; MRP2 multidrug resistance-associated protein 2; OATP, organic anion transporting polypeptide; P-gp, P-glycoprotein; PXR, pregnane X receptor; Q3GA, quercetin-3-O-β-D-glucoside; SULT, sulfotransferase; UGT, UDP glucuronosyltransferase; VER, verapamil.

Article Snippet: A rabbit anti-SULT1A1 polyclonal antibody (1:1,000; cat. no. bs-6283R) was purchased from Bioss (Beijing, China).

Techniques: Western Blot, Expressing, Standard Deviation